Determination of trimethylamine, trimethylamine N-oxide, and taurine in human plasma and urine by UHPLC-MS/MS technique

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Determination of trimethylamine, trimethylamine N-oxide, and taurine in human plasma and urine by UHPLC-MS/MS technique

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作者：Hussain Mohamad Awwad^a ; ^{s9huawwa@stud.uni-saarland.de" class="auth_mail" title="E-mail the corresponding author} ; Juergen Geisel^a ; Rima Obeid^a ; ^b ; ^{rima.obeid@uks.eu" class="auth_mail" title="E-mail the corresponding author}
关键词：CVs ; coefficients of variation ; FMO3 ; flavin-containing monooxygenase 3 ; IDL ; instrumental detection limit ; IQL ; instrumental quantification limit ; LOD ; limit of detection ; LOQ ; limit of quantification ; MRM ; multiple reaction monitoring ; TMA ; trimethylamine ; TMAO ; trimethylamine N-oxide ; UHPLC&ndash ; MS/MS ; ultrahigh performance liquid chromatography-tandem mass spectrometry
刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
出版年：2016
出版时间：1 December 2016
年：2016
卷：1038
期：Complete
页码：12-18
全文大小：736 K

文摘

•: We developed a UHPLC–MS/MS method for measurements of TMA, TMAO, and taurine in biological samples.
•: We studied the effect of pre-analytical conditions (blood material, storage, acidification) on the metabolites.
•: The effect of renal dysfunction on plasma and urinary markers was investigated.

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