Calibrated kallikrein generation in human plasma

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Calibrated kallikrein generation in human plasma

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作者：D. Biltoft^a ; ^b ; ^{dbiltoft@health.sdu.dk" class="auth_mail" title="E-mail the corresponding author} ; J.J. Sidelmann^a ; ^b ; L.F. Olsen^c ; Y. Palarasah^a ; ^b ; J. Gram^a ; ^b
关键词：PK ; prekallikrein ; FXI ; factor XI ; HMWK ; high molecular weight kininogen ; FXII ; factor XII ; FXIIa ; activated factor XII ; C1-inhibitor ; complement C1-esterase inhibitor ; α2M ; α2-macroglobulin ; CAT-method ; the calibrated automated thrombin generation method ; NaN3 ; sodium azide ; BSA ; bovine serum albumin ; PBS ; phosphate-buffered saline ; EDTA ; ethylenediaminetetraacetic acid ; FXII DEP ; factor XII depleted plasma ; PK DEP ; prekallikrein depleted plasma ; HAE ; hereditary angioedema ; CTI ; corn trypsin in
刊名：Clinical Biochemistry
出版年：2016
出版时间：October 2016
年：2016
卷：49
期：15
页码：1188-1194
全文大小：655 K

文摘

•: New global method for measuring kallikrein generation potential in plasma
•: Fluorogenic substrate Boc-LKR-AMC was specific for kallikrein activity.
•: Signal was externally calibrated with α₂M-kallikrein complexes.
•: Method detects abnormalities of the contact activation system.

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