A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents

详细信息    查看全文

• 作者：Isabel Leung¹ ; ² ; Nick Jarvik² ; Sachdev S. Sidhu¹ ; ² ; ^{sachdev.sidhu@utoronto.ca}
• 关键词：Ubv ; ubiquitin variant ; Ub ; ubiquitin ; wt ; wild-type ; EGFR ; epidermal growth factor receptor ; EGF ; epidermal growth factor ; Her3 ; human epidermal growth factor receptor 3 ; NRG ; neuregulin ; Grb2 ; growth factor receptor-bound protein 2 ; GST ; glutathione S-transferase ; SPR ; surface plasmon resonance ; IP ; immunoprecipitation ; Grb2-SH2 ; Grb2 SH2 domain ; p-Tyr ; phospho-tyrosine ; pERK ; phosphorylated ERK ; pMEK ; phosphorylated MEK ; ERK ; extracellular signal-regulated kinase ; MEK ; MAPK/ERK kinase
• 刊名：Journal of Molecular Biology
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：429
• 期：1
• 页码：115-127
• 全文大小：1845 K
• 卷排序：429

文摘

There is a great need for affinity reagents that can target proteins inside cells. We constructed a naïve library containing billions of Ubvs. We generated Ubvs that targeted the extracellular receptor human epidermal growth factor receptor 3. We generated Ubvs that inhibited the intracellular adapter Grb2. The naïve Ubv library can target diverse proteins inside cells.