Higher plant RecA-like protein is homologous to RadA

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• 作者：Toyotaka Ishibashi ; Minako Isogai ; Hiroyuki Kiyohara ; Masahiro Hosaka ; Hiroyuki Chiku ; Asami Koga ; Taichi Yamamoto ; Yukinobu Uchiyama ; Yoko Mori ; Junji Hashimoto et al.
• 关键词：RadA ; Plant DNA repair ; Rice ; RecA-like
• 刊名：DNA Repair
• 出版年：2006
• 出版时间：5 January 2006
• 年：2006
• 卷：5
• 期：1
• 页码：80-88
• 全文大小：448 K

文摘

A novel RecA-like protein, differing from Dmc1 and Rad51, was characterized in Oryza sativa L. cv. Nipponbare. Because the protein is homologous to bacterial RadA, the gene was designated OsRadA. The open reading frame was predicted to encode a 66 kDa protein of 619 amino acid residues and was found in plants but not animals or yeast. OsRadA showed D-loop and single-stranded DNA-dependent ATPase activities. Gene expression was found to be high in meristematic tissues, and was localized in the nucleus. An RNAi mutant of Arabidopsis thaliana RadA (AtRadA) was sensitive to mutagenic agents such as UV and MMC, suggesting that RadA functions in DNA repair.