Pregnancy outcome in mares following insemination deep in the uterine horn with low numbers of sperm selected by glass wool/Sephadex filtration, Percoll separation or absolute number

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• 作者：Nie ; Gary J. ; Johnson ; Kristina E. ; Wenzel ; James G.W.
• 关键词：Deep-horn insemination ; DHI ; Glass wool/Sephadex ; Percoll ; Sperm ; Mare
• 刊名：Animal Reproduction Science
• 出版年：2003
• 出版时间：November 20, 2003
• 年：2003
• 卷：79
• 期：1-2
• 页码：103-109
• 全文大小：56.5 K

文摘

Mares were inseminated deep in the uterine horn with 25 million sperm selected by glass wool/Sephadex (GWS) filtration, Percoll separation (PS) or absolute number (AN). Deep-horn insemination using a low-volume, smooth tipped, flexible pipette/catheter delivery system allowed more efficient use of stallion sperm and reduced post-breeding uterine reaction in mares. Mares were pregnant in 15/30, 13/30 and 10/30 cycles for GWS, PS and AN selection methods, respectively. Sperm selection method did not effect pregnancy outcome (P=0.422). However, sperm selected for deep-horn insemination by filtration through a glass wool/Sephadex column tended to improve fertility over simply using an absolute number of sperm (P=0.105).