Immobilization of laccase on gold, silver and indium tin oxide by zirconium–phosphonate–carboxylate (ZPC) coordination chemistry

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• 作者：M. Mazur ; P. Krysi&#x144 ; ski ; A. Michota-Kami&#x144 ; ska ; J. Bukowska ; J. Rogalski ; G.J. Blanchard
• 关键词：Zirconium phosphonate/carboxylate ; Laccase immobilization
• 刊名：Bioelectrochemistry
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：71
• 期：1
• 页码：15-22
• 全文大小：433 K

文摘

In this paper we present a simple method allowing for stable laccase immobilization on various conducting surfaces that retains the activity of the enzyme. The strategy for laccase immobilization presented in this paper relies on Zr⁴⁺ ion coordination chemistry that involves –COO⁻ terminal groups present on the protein. Using a host of techniques, including surface plasmon resonance (SPR), quartz crystal microbalance (QCM) gravimetry, atomic force microscopy (AFM), surface enhanced Raman scattering (SERS), resonance Raman scattering (RR) and electrochemical techniques, we show that laccase bound to a surface coordinatively through zirconium phosphonate/carboxylate (ZPC) functionalities forms a stable enzymatic layer with the enzyme retaining its activity to a significant extent.