Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping

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Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping

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作者：Yong Tae Kim^a ; Dohwan Lee^a ; Hyun Young Heo^a ; Jeong Eun Sim^b ; Kwang Man Woo^b ; Do Hyun Kim^a ; Sung Gap Im^a ; Tae Seok Seo^a ; ^{seots@kaist.ac.kr" class="auth_mail" title="E-mail the corresponding author}
关键词：Integrated microdevice ; Solid phase DNA extraction ; Polymerase chain reaction ; Capillary electrophoresis ; Mini Y STR typing
刊名：Biosensors and Bioelectronics
出版年：2016
出版时间：15 April 2016
年：2016
卷：78
期：Complete
页码：489-496
全文大小：2173 K

文摘

•: We developed an integrated slidable SPE-μPCR-μCE microchip for forensic genotyping.
•: The slidable chip enables to get rid of microvalves and tubing for fluidic control.
•: Mini Y STR profiles could be identified from 1 μL human whole blood within 60 min.
•: Mini Y STR typing could be executed with the female and male blood mixture sample.

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