Orthogonal spin labeling using click chemistry for in vitro and in vivo applications

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• 作者：Svetlana Kucher^a ; Sergei Korneev^b ; Swati Tyagi^c ; Ronja Apfelbaum^d ; Dina Grohmann^d ; ¹ ; Edward A. Lemke^c ; Johann P. Klare^a ; Heinz-Jü ; rgen Steinhoff^a ; ^{hsteinho@uos.de} ; Daniel Klose^a ; ^{daniel.klose@phys.chem.ethz.ch}
• 关键词：Site-directed spin labeling ; EPR spectroscopy ; Orthogonal labeling ; Click chemistry ; DEER ; In vivo EPR
• 刊名：Journal of Magnetic Resonance
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：275
• 期：Complete
• 页码：38-45
• 全文大小：1180 K
• 卷排序：275

文摘

Site-directed spin labeling of unnatural amino acids in proteins by click chemistry. Quantitative labeling yields within incubation times as short as 30 min. Orthogonal labeling approach independent of cysteines. Evaluation of Cu-free- and Cu-catalyzed reaction variants. Suitable coupling chemistry for spin labeling in vivo.