Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies

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• 作者：Isaac Vizcaino-Caston¹ ; ³ ; Catherine A. Kelly² ; Annabel V.L. Fitzgerald² ; Gary A. Leeke¹ ; Mike Jenkins² ; Tim W. Overton¹ ; ³ ; ^{t.w.overton@bham.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Polyhydroxyalkanoate ; Dimethyl sulphoxide ; Solvent extraction ; Bioplastic
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：121
• 期：1
• 页码：101-104
• 全文大小：724 K

文摘

We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.