Can currently available non-animal methods detect pre and pro-haptens relevant for skin sensitization?

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• 作者：Grace Patlewicz^a ; ^{patlewicz.grace@epa.gov} ; Silvia Casati^b ; David A. Basketter^c ; David Asturiol^b ; David W. Roberts^d ; Jean-Pierre Lepoittevin^e ; Andrew P. Worth^b ; Karin Aschberger^b
• 关键词：Adverse Outcome Pathway ; Skin sensitization ; Pre-hapten ; Pro-hapten ; DPRA ; h-CLAT ; KeratinoSens&trade ; Reaction mechanistic domain
• 刊名：Regulatory Toxicology and Pharmacology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：82
• 期：Complete
• 页码：147-155
• 全文大小：1159 K
• 卷排序：82

文摘

The three most established non-animal test methods for skin sensitization are the DPRA, the KeratinoSens™ and the h-CLAT. A dataset of 127 substances for which outcomes were available in all three non-animal tests and the LLNA was collected. 28 substances required activation to be sensitizing and were correctly identified by 1 or more of the test methods. Six substances were categorized as pro-haptens exclusively but were still correctly identified by a cell based assay. Skin metabolism was not a major consideration for assessing skin sensitization potential in this dataset.