Imaging the Photosystem I/Photosystem II chlorophyll ratio inside the leaf

详细信息    查看全文

• 作者：Emilie Wientjes^a ; ^{emilie.wientjes@wur.nl} ; John Philippi^a ; Jan Willem Borst^b ; ^c ; Herbert van Amerongen^a ; ^c
• 关键词：Microscopy ; Fluorescence lifetime imaging ; Light-harvesting complex II ; LHCII
• 刊名：Biochimica et Biophysica Acta (BBA) - Bioenergetics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1858
• 期：3
• 页码：259-265
• 全文大小：1726 K
• 卷排序：1858

文摘

The PSI/PSII chlorophyll (Chl) ratio is measured in leafs with lifetime microscopy. The PSI/PSII Chl ratio is different for the top and bottom of the leaf. Far red-light acclimation reduces the PSI/PSII Chl ratio by a factor of three. The PSI/PSII Chl ratio was visualized within grana and stroma inside the leaf. The method can be used to follow dynamic processes in chloroplasts.