A report on extensive lateral genetic reciprocation between arsenic resistant Bacillus subtilis and Bacillus pumilus strains analyzed using RAPD-PCR

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• 作者：Sapna Khowal^a ; Md. Zulquarnain Siddiqui^a ; ¹ ; Shadab Ali^a ; ¹ ; Mohd. Taha Khan^a ; ¹ ; Mather Ali Khan^b ; Samar Husain Naqvi^c ; Saima Wajid^a ; ^{swajid@jamiahamdard.ac.in} ; ^{wajid_saima@hotmail.com}
• 关键词：As ; arsenic ; CTAB ; cetyltrimethylammonium bromide ; DDW ; double distilled water ; ITS ; Internal Transcribed Spacer Regions ; gltX ; glutamate-tRNA ligase ; HGT ; horizontal gene transfer ; rDNA ; ribosomal DNA ; mM ; milli molar ; MTC ; maximum tolerable concentration ; ppb ; parts per billion ; RAPD ; random amplified polymorphic DNA ; rpoB ; DNA-directed RNA polymerase subunit beta ; SCAR ; Sequenced Characterized Amplified Region ; TAE ; Tris-acetate-EDTA
• 刊名：Molecular Phylogenetics and Evolution
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：107
• 期：Complete
• 页码：443-454
• 全文大小：1353 K
• 卷排序：107

文摘

The study involves isolation and characterization of arsenic resistant bacteria from soil. The phylogenetic consanguinity among isolates was studied. RAPD-PCR technique was used to analyze genetic similarity between isolates. Bacillus subtilis and Bacillus pumilus strains may exhibit extensive horizontal gene transfer. Arsenic resistant potency in Bacillus sonorensis and high arsenite tolerance in Bacillus pumilus strains was identified.