Improved biodegradation of synthetic azo dye by horseradish peroxidase cross-linked on nano-composite support

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• 作者：Huaiyan Sun ; Xinyu Jin ; Nengbing Long ; Ruifeng Zhang ; ^{zhangruifeng@nbu.edu.cn}
• 关键词：Horseradish peroxidase ; Nano-composites support ; Epoxy cross-linker ; Azo dye ; Decolorization
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：1049-1055
• 全文大小：1511 K
• 卷排序：95

文摘

A novel ZnO nanowires/macroporous SiO2 composite support was used to immobilize horseradish peroxidase through in-situ cross-linking method using long-spaced epoxy cross-linker. The formed nanobiocatalyst can provide many block-free channels for mass transfer and allow the cross-linked HRP to take a proper orientation for exposing of active site. The positive-charged ZnO nanowires played an important role in promoting both the immobilization of HRP and the reaction between HRP and dyes. Immobilized HRP exhibited satisfied efficiency in the decolorization of azo dyes. The storage stability and reusability of HRP were greatly improved through the immobilization.