Genetic stability of an Escherichia coli strain engineered to produce a novel therapeutic DNA vaccine encoding chicken type II collagen for rheumatoid arthritis

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• 作者：Long Juan¹ ; Zhao Xiao¹ ; Yuan Fang ; Liang Fei ; Liu Nan ; Yun Song ; Wang Ling ; Sun Yuying ; Xi Yongzhi ; ^{xiyz@yahoo.com}
• 关键词：Therapeutic DNA vaccine ; Chicken type II procollagen ; Engineered Escherichia coli ; Genetic stability ; Three-tier seed bank
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：86-93
• 全文大小：1289 K
• 卷排序：52

文摘

A DH5α strain producing CCII DNA vaccine was used to found a three-tier cell bank. Genetic stability of this strain during continue passage and storage was proved. This strain showed a high plasmid preservation and yield after long-term storage.