A G-quadruplex-binding macrodomain within the 鈥淪ARS-unique domain鈥?is essential for the activity of the SARS-coronavirus replication-transcription complex

设为首页

收藏本站

网站地图 | English | 公务邮箱

About the library

Background
History
Leadership
Organization

Readers' Guide

Opening Hours
Collections
Help Via Email

Publications

Electronic Information Resources

A G-quadruplex-binding macrodomain within the 鈥淪ARS-unique domain鈥?is essential for the activity of the SARS-coronavirus replication-transcription complex

详细信息查看全文

作者：Yuri Kusov^a ; ^b ; ^{koussov@biochem.uni-luebeck.de" class="auth_mail" title="E-mail the corresponding author} ; Jinzhi Tan^a ; ^{tanjinzhi@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Enrique Alvarez¹ ; ^c ; ^{ealvarezmba@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Luis Enjuanes^c ; ^{L.enjuanes@cnb.csic.es" class="auth_mail" title="E-mail the corresponding author} ; Rolf Hilgenfeld^a ; ^b ; ^{hilgenfeld@biochem.uni-luebeck.de" class="auth_mail" title="E-mail the corresponding author}
关键词：SARS-CoV replicon ; SARS-unique domain ; Macrodomain ; X-domain ; Reverse genetics ; G-quadruplex ; MERS-CoV
刊名：Virology
出版年：2015
出版时间：October 2015
年：2015
卷：484
期：Complete
页码：313-322
全文大小：1620 K

文摘

•: A SARS-CoV replicon encoding Renilla luciferase as reporter protein is constructed.
•: The role of three macrodomains for the replication/transcription complex is analyzed.
•: In contrast to macrodomains X and SUD-N, SUD-M is found indispensable for replication.
•: Site-directed mutagenesis identifies charged SUD-M residues required for replication.
•: These residues have previously been shown to be involved in G-quadruplex binding.