Development and validation of a liquid chromatography-MS/MS method for simultaneous quantification of tenofovir and efavirenz in biological tissues and fluids

详细信息    查看全文

• 作者：Luisa Barreiros^a ; ^{lbarreiros@ff.up.pt} ; Cassilda Cunha-Reis^b ; Eduarda M.P. Silva^a ; Joana R.B. Carvalho^a ; José ; das Neves^b ; ^c ; Bruno Sarmento^b ; ^c ; Marcela A. Segundo^a
• 关键词：Tenofovir ; Efavirenz ; Antiretrovirals ; Intravaginal administration ; Pre-exposure prophylaxis
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 March 2017
• 年：2017
• 卷：136
• 期：Complete
• 页码：120-125
• 全文大小：644 K
• 卷排序：136

文摘

Quantification of tenofovir (TFV) and efavirenz (EFV) was attained for 4–500 ng mL−1. Validated LC–MS/MS method for vaginal tissue, lavage and blood plasma was developed. Matrix effects were observed for EFV in tissue (+17%) and plasma (−50%) extracts. LOD and LOQ values were ≤0.4 and ≤0.7 ng mL−1 in sample extracts. PK profile after intravaginal administration showed low systemic exposure.