Pbcrk-1, the Plasmodium berghei orthologue of P. falciparum cdc-2 related kinase-1 (Pfcrk-1), is essential for completion of the intraerythrocytic asexual cycle

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• 作者：Radha Rangarajan ; Amy Bei ; Natohya Henry ; Marylin Madamet ; Daniel Parzy ; Marie-Paule Nivez ; Christian Doerig and Ali Sultan
• 刊名：Experimental Parasitology
• 出版年：2006
• 出版时间：March 2006
• 年：2006
• 卷：112
• 期：3
• 页码：202-207
• 全文大小：320 K

文摘

The molecular mechanisms underlying gametocytogenesis in malaria parasites are not understood. Plasmodium falciparum cdc2-related kinase 1 (pfcrk-1), a gene that is expressed predominantly in gametocytes, bears homology to the PITSLRE subfamily of cyclin-dependent kinases and has been hypothesized to function as a negative regulator of the cell cycle. We attempted to knock-out pbcrk-1, the P. berghei orthologue of pfcrk-1, but were unable to recover P. berghei parasites with a disrupted pbcrk-1 locus. In contrast, an integration event at this locus that did not result in a loss-of-function of the pbcrk-1 gene was readily observed. This strongly suggests that a functional pbcrk-1 gene product is essential to intraerythrocytic asexual multiplication.