Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion

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• 作者：Aaron F. Phillips¹ ; ⁴ ; Armê ; l R. Millet² ; ³ ; ⁴ ; Marco Tigano¹ ; Sonia M. Dubois² ; Hannah Crimmins¹ ; Loelia Babin² ; ³ ; Marine Charpentier² ; Marion Piganeau² ; Erika Brunet² ; ³ ; ^{erika.brunet@inserm.fr} ; Agnel Sfeir¹ ; ⁵ ; ^{agnel.sfeir@med.nyu.edu}
• 关键词：mtDNA ; common deletion ; replication ; repair ; mito-TALEN ; mito-SMARD ; microhomology-mediated end joining
• 刊名：Molecular Cell
• 出版年：2017
• 出版时间：2 February 2017
• 年：2017
• 卷：65
• 期：3
• 页码：527-538.e6
• 全文大小：2862 K
• 卷排序：65

文摘

Development of mito-SMARD, a single-molecule approach to study mtDNA replication In vivo analysis validates the strand displacement mode of mtDNA replication Twinkle helicase mutations promote fork stalling near the common deletion Repair of mito-TALEN-induced breaks triggers the common deletion