文摘
A new species, Isospora anthochaerae n. sp. is described from a Red wattlebird (Anthochaera carunculata). Sporulated oocysts (n = 37) are subspherical, with smooth colourless to pale brown bilayered oocyst wall, 0.8 渭m thick (outer layer 0路6 渭m, inner 0.2 渭m thick). Oocyst with 2 spheroidal to subspheroidal sporocysts. Oocyst length, 23.4 渭m (20.0-26.0); oocyst width, 20.7 渭m (19.0-22.0); oocyst length/width (L/W) ratio, 1.1. Micropyle, oocyst residuum and polar granule are absent. Sporocysts with compact sporocyst residuum and 4 sporozoites. Sporocyst length, 14.5 渭m; sporocyst width, 10.1 渭m sporocyst L/W ratio, 1.4. Molecular analysis was conducted at four loci; the ribosomal internal transcribed spacer (ITS), the 18S and 28S ribosomal RNA and the mitochondrial cytochrome oxidase gene (COI). At the COI locus, I. anthochaerae n. sp. exhibited 98.5% similarity to Isospora lesouefi from a Regent honeyeater (Xanthomyza phrygia) and 98% similarity with an Isospora sp. (iSAT5) from a blackcap (Sylvia atricapilla). Based on morphological and molecular data, this isolate is a new species of coccidian parasite that to date has only been found in Red wattlebirds.
