Isolation of PCR ready-human DNA using copper nanoparticles from skeletal remains

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• 作者：Anand Lodha^a ; ^{forensicwithanand@gmail.com} ; Niha Ansari^a ; ^{n.ansari3@gmail.com} ; Shahil Shah^b ; M.V. Rao^b ; Shobhana K. Menon^a ; ^{shobhanamenon07@gmail.com}
• 关键词：DNA extraction ; Skeletal remains ; Blood ; Copper nanoparticles ; Polymerase chain reaction ; Forensic science
• 刊名：Forensic Science International
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：270
• 期：Complete
• 页码：146-152
• 全文大小：1481 K
• 卷排序：270

文摘

A novel approach of PCR ready-human DNA extraction method from skeletal remains. Rapid, cost effective, sensitive and non-hazardous method. Required a very small quantities of blood and skeletal remains. Does not require hazardous organic solvents or expensive enzyme treatment. First time, one-step green rout synthesized copper nanoparticles (CuNPs) is applied.