Characterization of fatty acid elongase enzymes from germinating pea seeds

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• 作者：Barrett ; Philippa B. ; Harwood ; John L.
• 关键词：Pisum sativum ; Leguminosae ; germinating seeds ; very-long-chain fatty acid synthesis ; microsomal fractions ; fatty acid elongation ; behenate ; arachidate
• 刊名：Phytochemistry
• 出版年：1998
• 出版时间：August, 1998
• 年：1998
• 卷：48
• 期：8
• 页码：1295-1304
• 全文大小：708 K

文摘

In vitro biosynthesis of radioactive arachidate and behenate was observed when microsomal fractions of germinating pea seeds were incubated with exogenous stearoyl-CoA (18:0-CoA) or arachidoyl-CA (20:0-CoA) in the presence of NADPH, [2-¹⁴C]malonyl-CoA and ATP. Characterization of parameters required for optimal stearoyl- and arachidoyl-CoA elongation revealed that, at least, two chain-length-specific elongases are necessary for very-long-chain fatty acid synthesis. Both enzymes were found to be sensitive to the group-selective reagents, p-CMB, NEM, iodoacetate, arsenite and phenylglyoxal. Subcellular fractionation studies indicated that both of these elongases were localized mainly in the endoplasmic reticulum.