Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via <sup>13</sup>C labeling experiments

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Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via ¹³C labeling experiments

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作者：Ming Hong ; Mingzhi Huang ; ^{huangmz@ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ju Chu ; ^{juchu@ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Yingping Zhuang ; Siliang Zhang
关键词：FL ; fractional labeling ; EMP ; Embden-Meyerhof-Parnas ; ED ; Entner-Doudoroff ; PP ; pentose phosphate ; TCA ; tricarboxylic acid cycle ; MFA ; metabolic flux analysis ; SSR ; sum of the squared residuals ; GAP ; glyceraldehyde-3-phosphate ; G6P ; glucose 6- phosphate ; Ru5P ; ribulose 5- phosphate ; OAC ; oxaloacetate ; Cit ; Citrate ; 6PG ; 6-phosphate gluconate ; KDPG ; 2-Keto-3-deoxy-6-phosphogluconate ; F6P ; fructose 6-phosphate ; FBP ; fructose 1 ; 6-biphosphate ; AKG ; 2-oxoglutarate ; Glu ; glutamate ; 3PG ; glycerate 3-ph
刊名：Journal of Biotechnology
出版年：2016
出版时间：10 August 2016
年：2016
卷：231
期：Complete
页码：1-8
全文大小：1682 K

文摘

•: Very low growth rate of S. erythraea in minimal medium.
•: Low activity of EMP pathway and high activity of ED pathway for glucose utilization.
•: Promotion on cell growth of proline and use of proline as building block.
•: Proline addition relieved the energy burden for cell growth.

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