Cell-type dependent response to photodynamic treatment in 3D collagen cell cultures

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• 作者：Anton Matea&scaron ; í ; k^a ; Michal Trnka^b ; Karol Kajo^c ; Miroslava Vallová ; ^c ; Beata Čunderlí ; ková ; ^a ; ^b ; ^beata@ilc.sk
• 关键词：2D ; two-dimensional ; 3D ; three-dimensional ; ALA ; aminolevulinic acid ; AO ; Acridine Orange ; CMO ; CellMask Orange ; ECM ; extracellular matrix ; FDA ; Food and Drug Administration agency ; PDT ; photodynamic therapy ; PpIX ; protoporphyrin IX ; UV ; ultraviolet ; VIS ; visible
• 刊名：Journal of Photochemistry and Photobiology B: Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：166
• 期：Complete
• 页码：94-103
• 全文大小：2170 K
• 卷排序：166

文摘

Cell response to ALA-PDT was cell-type dependent in cell monolayers and 3D collagen cultures. ALA-PDT effect evaluated by WST-1 assay did not correlate with modified colony assay in Het-1A cells. ALA-PDT killed Kyse 450, but not Kyse 70 or Het-1A cells in 3D collagen gels, if analyzed 24 h after PDT. ALA induced PpIX fluorescence was detected in 3D collagen gels of Kyse 450, not Kyse 70 or Het-1A cells.