Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis

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• 作者：Shinya Kaneko ; Takashi Takeuchi ; Mitsuhiro Itaya
• 关键词：Genome vector ; Recombination ; Transformation ; Mouse genome
• 刊名：Journal of Biotechnology
• 出版年：2009
• 出版时间：5 February 2009
• 年：2009
• 卷：139
• 期：3
• 页码：211-213
• 全文大小：532 K

文摘

A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.