Reprogramming of Mouse and Human Cells to Pluripotency Using Mature MicroRNAs

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• 作者：Norikatsu Miyoshi¹ ; Hideshi Ishii¹ ; ² ; ⁴ ; ^{hishii@gesurg.med.osaka-u.ac.jp"" rel=""nofollow} ; Hiroaki Nagano¹ ; Naotsugu Haraguchi¹ ; Dyah  ; Laksmi Dewi¹ ; Yoshihiro Kano¹ ; Shinpei Nishikawa¹ ; Masahiro Tanemura¹ ; Koshi Mimori² ; Fumiaki Tanaka² ; Toshiyuki Saito³ ; Junichi Nishimura¹ ; Ichiro Takemasa¹ ; Tsunekazu Mizushima¹ ; Masataka Ikeda¹ ; Hirofumi Yamamoto¹ ; Mitsugu Sekimoto¹ ; Yuichiro Doki¹ ; Masaki Mori¹ ; ² ; ⁴ ; ^{mmori@gesurg.med.osaka-u.ac.jp"" rel=""nofollow}
• 刊名：Cell Stem Cell
• 出版年：2011
• 出版时间：3 June 2011
• 年：2011
• 卷：8
• 期：6
• 页码：633-638
• 全文大小：792 K

文摘

Summary

Induced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.