Hydrophilic interaction chromatography-multiple reaction monitoring mass spectrometry method for basic building block analysis of low molecular weight heparins prepared through nitrous acid depolymerization

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• 作者：Xiaojun Sun^a ; ^b ; Zhimou Guo^c ; ^{guozhimou@dicp.an.cn} ; Mengqi Yu^a ; Chao Lin^d ; Anran Sheng^a ; Zhiyu Wang^e ; Robert J. Linhardt^b ; Lianli Chi^a ; ^{lianlichi@sdu.edu.cn}
• 关键词：Hydrophilic interaction chromatography ; Low molecular weight heparin ; Basic building blocks ; LC-MRM-MS/MS
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：1479
• 期：Complete
• 页码：121-128
• 全文大小：2570 K
• 卷排序：1479

文摘

The new LC-MRM-MS/MS method can detect the most building blocks of LMWHs. A maltose modified HILIC column offered the best separation. The nitrous acid depolymerized LMWHs possess characteristic basic building blocks. The chemically modified minor components should be carefully examined.