Ceramide signaling targets the PP2A-like protein phosphatase Sit4p to impair vacuolar function, vesicular trafficking and autophagy in Isc1p deficient cells

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Ceramide signaling targets the PP2A-like protein phosphatase Sit4p to impair vacuolar function, vesicular trafficking and autophagy in Isc1p deficient cells

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作者：Vitor Teixeira^a ; ^b ; ^c ; Tâ ; nia C. Medeiros^b ; Rita Vilaç ; a^a ; ^b ; Joã ; o Ferreira^b ; Pedro Moradas-Ferreira^a ; ^b ; ^c ; Ví ; tor Costa^a ; ^b ; ^c ; ^{vcosta@ibmc.up.pt" class="auth_mail" title="E-mail the corresponding author}
关键词：CAPP ; Ceramide-activated Protein Phosphatase ; CLS ; chronological lifespan ; CPY ; carboxypeptidase Y ; Cvt ; cytoplasm-to-vacuole targeting ; DMSO ; dimethyl sulfoxide ; DTT ; dithiothreitol ; EDTA ; ethylenediamine tetraacetic acid ; ER ; endoplasmic reticulum ; FACS ; fluorescence-activated cell sorting ; GFP ; green fluorescent protein ; HOG ; high osmolarity glycerol ; MOPS ; 3-(N-morpholino)propanesulfonic acid ; PAS ; phagophore assembly site ; PBS ; phosphate buffer saline ; PCR ; polymerase chain reaction ; PDS ; p
刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
出版年：2016
出版时间：January 2016
年：2016
卷：1861
期：1
页码：21-33
全文大小：1264 K

文摘

•: The impact of ceramide signaling on vacuolar function and autophagy was studied.
•: Isc1p (sphingomyelinase) deficiency impairs vacuolar structure and functions.
•: The isc1Δ cells exhibit defective vesicular trafficking and autophagy.
•: These phenotypes are mediated by the ceramide-activated protein phosphatase Sit4p.
•: Downregulation of TORC1–Sch9p signaling also suppresses isc1Δ phenotypes.