We investigated a novel surface modification to mildly regenerate SPR sensor chip.

This was attained by a new binder changing the used capture antibody with fresh one.

Analysis of multiple samples using the same sensor was reproducible at a rate >98.7%.

The dose-response curve was linear for CK-MB, with an acceptable CV <8.8%.

CK-MB was semi-continuously monitored on the same sensor up to 30 days.