Immobilized fusion protein affinity chromatography combined with HPLC-ESI-Q-TOF-MS/MS for rapid screening of PPARγ ligands from natural products

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• 作者：Junfeng Zhu ; Xiaojiao Yi ; Wenhui Liu ; Yingchun Xu ; Shuqing Chen ; Yongjiang Wu ; ^{yjwu@zju.edu.cn}
• 关键词：Peroxisome proliferator-activated receptor-γ ; Immobilized fusion protein affinity chromatography ; HPLC&ndash ; ESI-Q-TOF-MS/MS ; Multicomponent screening ; Natural products
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：508-515
• 全文大小：1043 K
• 卷排序：165

文摘

A novel affinity selection combined with LC/MS approach was developed for multicomponent screening. The proposed screening method is time- and cost-effective, easily recycled, and high-throughput. Isochlorogenic acid A in Dendranthema indicum flowers was identified as a PPARγ ligand for the first time.