Azaspiracid: First evidence of protein binding in shellfish

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• 作者：K.J. Nzoughet ; J.T.G. Hamilton ; S.D. Floyd ; A. Douglas ; J. Nelson ; L. Devine ; C.T. Elliott
• 关键词：Azaspiracids (AZAs) ; Isoelectric focusing (IEF) ; Size exclusion chromatography (SEC) ; Sodium docecyl sulphate– ; polyacrylamide gel electrophoresis (SDS– ; PAGE) ; Liquid chromatography coupled with tandem mass spectrometry analysis (LC– ; MS/M
• 刊名：Toxicon
• 出版年：2008
• 出版时间：1 June 2008
• 年：2008
• 卷：51
• 期：7
• 页码：1255-1263
• 全文大小：679 K

文摘

The occurrence of azaspiracid (AZA) toxins in contaminated shellfish has been the focus of much research. The present study investigated the binding properties of these toxins in mussels of the species Mytilus edulis. The work involved extraction of proteins and AZAs from contaminated mussel hepatopancreas and examination of the extracts by isoelectric focusing (IEF), size exclusion chromatography (SEC) and sodium docecyl sulphate–polyacrylamide gel electrophoresis (SDS–PAGE). Liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) was also performed in this study to identify AZAs. Blank mussels were subjected to the same purification and analytical procedures.