Measuring propionyl-CoA carboxylase activity in phytohemagglutinin stimulated lymphocytes using high performance liquid chromatography

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Measuring propionyl-CoA carboxylase activity in phytohemagglutinin stimulated lymphocytes using high performance liquid chromatography

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作者：Yu-Ning Liu^a ; Tze-Tze Liu^a ; ^b ; Ya-Ling Fan^a ; Dau-Ming Niu^c ; Yin-Hsiu Chien^d ; Yen-Yin Chou^e ; Ni-Chung Lee^d ; Kwang-Jen Hsiao^a ; ^f ; Yen-Hui Chiu^a ; ^{yhchiu@ntu.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; ^{A3360@tpech.gov.tw" class="auth_mail" title="E-mail the corresponding author}
关键词：FAD ; flavin adenine dinucleotide ; FBS ; fetal bovine serum ; IV-CoA ; isovaleryl-CoA ; IVD ; isovaleryl-CoA dehydrogenase ; MC-CoA ; methylcrotonyl-CoA ; MM-CoA ; methylmalonyl-CoA ; PA ; propionic academia ; PCC ; propionyl-CoA carboxylase ; PHA ; phytohemagglutinin ; PMS ; phenazinemethosulfate ; PP-CoA ; propionyl-CoA
刊名：Clinica Chimica Acta
出版年：2016
出版时间：30 January 2016
年：2016
卷：453
期：Complete
页码：13-20
全文大小：739 K

文摘

•: A nonradiometric and reliable manipulated method used for measuring PCC activity
•: PHA-stimulated lymphocytes were recommended as the subjects for PCC assay.
•: The PCC assay only requires 0.5 to 1.0 ml of whole blood from newborns.
•: This method is applicable for analyzing samples from distant medical centers.

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