Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity

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• 作者：Benjaminas Valiauga^a ; Elsie M. Williams^b ; David F. Ackerley^b ; Narimantas Čėnas^a ; ^{narimantas.cenas@bchi.vu.lt}
• 关键词：NfsA ; Quinones ; Nitroaromatic compounds ; CB-1954 ; Bioreductive activation
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：614
• 期：Complete
• 页码：14-22
• 全文大小：1077 K
• 卷排序：614

文摘

E. coli NfsA is a highly promiscuous quinone and nitroaromatic oxidoreductase. Reactions follow a ping pong mechanism with the oxidative half-reaction rate-limiting. Reactivity of quinones or nitroaromatics correlates with one-electron reduction potential. Reactivity was generally lower for quinones than nitroaromatics having similar E17. Quinone reactivity pH dependence suggests a single-step hydride transfer mechanism.