Efficient induction of dopaminergic neuron differentiation from induced pluripotent stem cells reveals impaired mitophagy in PARK2 neurons

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• 作者：Sadafumi Suzuki^a ; Wado Akamatsu^b ; ^{awado@juntendo.ac.jp} ; Fumihiko Kisa^a ; Takefumi Sone^a ; Kei-ichi Ishikawa^b ; ^c ; Naoko Kuzumaki^d ; Hiroyuki Katayama^e ; Atsushi Miyawaki^e ; Nobutaka Hattori^c ; Hideyuki Okano^a ; ^{hidokano@a2.keio.jp}
• 关键词：Induced pluripotent stem cells ; Parkinson's disease ; Mitophagy ; Flow cytometry ; Dopaminergic neurons
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：29 January 2017
• 年：2017
• 卷：483
• 期：1
• 页码：88-93
• 全文大小：1550 K
• 卷排序：483

文摘

CD184high/CD44- sorting enriches for DA neurons during neural differentiation from hiPSCs. Highly purified DA neurons from PD-iPSCs stably reproduce PD phenotypes. The mt-mKeima reporter system allows visualization of impaired mitophagy in PARK2 DA neurons.