Nef modulates the immunogenicity of Gag encoded in a non-infectious HIV DNA vaccine

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• 作者：Geraldine Arrode ; Ramakrishna Hegde ; Yuhuai Jin ; Dinesh Kumar Singh ; Opendra Narayan ; Yahia Chebloune
• 关键词：HIV ; DNA vaccine ; Nef ; Apoptosis ; CD8 T cells
• 刊名：Vaccine
• 出版年：2008
• 出版时间：23 July 2008
• 年：2008
• 卷：26
• 期：31
• 页码：3795-3804
• 全文大小：3421 K

文摘

Gag-CD8⁺ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Δ4 Nef(+)), creating Δ5 Nef(−), would affect its immunogenicity. When compared with Δ4, mice injected with Δ5 developed significantly lower CD8⁺ T cell responses to Gag, but no significant change in the responses to Env was observed. In vitro, deletion of Nef abrogated the induced cell death, production of virus-like particles and release of Gag from transfected cells. Thus, the effect of Nef in causing extrusion of Gag might adjuvant the CD8⁺ T cell responses to Gag in DNA vaccine.