Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation

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Enrichment of hydrophobic membrane proteins using Triton X-114 and subsequent analysis of their N-glycosylation

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作者：Tamara Pavić^a ; Ivan Gudelj^b ; Toma Keser^a ; Maja Pučić-Baković^b ; Olga Gornik^a ; ^{ogornik@pharma.hr" class="auth_mail" title="E-mail the corresponding author}
关键词：N-glycosylation ; Membrane proteins ; Cloud-point extraction (CPE)
刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
出版年：2016
出版时间：August 2016
年：2016
卷：1860
期：8
页码：1710-1715
全文大小：768 K

文摘

•: A protocol for extraction of membrane proteins and analysis of their N-glycans is proposed.
•: Triton X-114 is a detergent that can be used for this purpose.
•: Detergent must be removed prior to HILIC-UPLC analysis of glycans.
•: Chloroform/methanol/water precipitation is optimal for detergent removal.
•: This protocol enables reproducible HILIC-UPLC analysis of N-glycans from enriched proteins.

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