Effect of 24-epibrassinolide on Brassica napus alternative respiratory pathway, guard cells movements and phospholipid signaling under salt stress

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• 作者：Michael Derevyanchuk^a ; Sergii Kretynin^a ; Oksana Iakovenko^a ; Raisa Litvinovskaya^c ; Vladimir Zhabinskii^c ; Jan Martinec^d ; Yaroslav Blume^b ; Vladimir Khripach^c ; Volodymyr Kravets^a ; ^{kravets@bpci.kiev.ua}
• 关键词：AOX ; alternative oxidase ; BRs ; brassinosteroids ; BRZ ; brassinazole ; COX ; cytochrome oxidase ; DAG ; diacylglycerol ; DGK ; diacylglycerol kinase ; DW ; dry weight ; EBL ; 24-epibrassinolide ; mETC ; mitochondrial electron transport chain ; NBD ; nitrobenzoxadiazole ; NEM ; N-ethylmaleimide ; NO ; nitric oxide ; PA ; phosphatidic acid ; PP2A ; protein phosphatase 2A ; ROS ; reactive oxygen species ; SHAM ; salicylhydroxamic acid ; TLC ; thin-layer chromatography
• 刊名：Steroids
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：117
• 期：Complete
• 页码：16-24
• 全文大小：2904 K
• 卷排序：117

文摘

Using Brassica napus roots we observed statistically significant increase in alternative respiratory pathway in response to exogenous 24-epibrassinolide (EBL) under optimal conditions and salinity. Also we observed activation of phospholipid signaling under the same conditions in response to EBL by measuring levels of lipid second messengers – diacylglycerol (DAG) and phosphatidic acid (PA). We found that brassinosteroids cause closure of stomata in isolated leaf disks while inhibitors of alternative oxidase cancelled these effects. This study demonstrates that BRs activate total respiration rate, alternative respiratory pathway, production of PA and DAG, stimulate stomata closure and growth under optimal conditions and salinity. Also, specific inhibitor of brassinosteroids biosynthesis decreased alternative respiratory pathway and production of lipid messengers in rape plants.