Association and insulin regulated translocation of hormone-sensitive lipase with PTRF

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• 作者：Nabila Aboulaich ; Unn Ö ; rtegren ; Alexander V. Vener and Peter Strå ; lfors
• 关键词：Hormone-sensitive lipase ; Polymerase I and transcript release factor ; Adipocyte ; Human ; Insulin ; Translocation ; Protein complex ; Caveolae ; Lipid metabolism ; Transcriptional control
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：24 November 2006
• 年：2006
• 卷：350
• 期：3
• 页码：657-661
• 全文大小：182 K

文摘

Polymerase I and transcript release factor (PTRF) is in human adipocytes mainly localized at the plasma membrane. This localization was under control of insulin, which translocated PTRF to the cytosol and nucleus, indicating a novel role for PTRF in insulin transcriptional control. In the plasma membrane PTRF was specifically bound to a triacylglycerol-metabolizing subclass of caveolae containing hormone-sensitive lipase (HSL). In response to insulin PTRF was translocated to the cytosol in parallel with HSL. PTRF and HSL were quantitatively immunoprecipitated from the cytosol by antibodies against either PTRF or HSL. The findings indicate also a novel extranuclear function for PTRF in the control of lipolysis.