Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase

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• 作者：Brian C. Pierce^a ; ^b ; ^{brian.pierce@dupont.com} ; ^{bpierce72@gmail.com} ; Jane Wittrup Agger^b ; ^{jaag@kt.dtu.dk} ; Jesper Wichmann^a ; ^{jesper.wichmann@dupont.com} ; Anne S. Meyer^b ; ^am@kt.dtu.dk
• 关键词：LPMO ; lytic polysaccharide monooxygenase ; MALDI ; matrix assisted laser desorption ionization ; HPAEC-PAD ; high performance anion exchange chromatography with pulsed amperometric detection ; PASC ; phosphoric acid swollen cellulose ; DP ; degree of polymerization
• 刊名：Enzyme and Microbial Technology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：98
• 期：Complete
• 页码：58-66
• 全文大小：1633 K
• 卷排序：98

文摘

T. reesei LPMO TrCel61A has oxidative activity on PASC and treated soy spent flake. TrCel61A has no activity on DP 5-6 cello-oligomers, galactan, and soy spent flake. TrCel61A shows C1 and C4 type oxidation with ESI–MS, MALDI-ToF, and HPAEC-PAD. Treatment with TrCel61A boosts endoglucanase hydrolytic depolymerization by 27%.