‘Yellow' laccase of Panus tigrinus oxidizes non-phenolic substrates without electron-transfer mediators

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• 作者：Leontievsky ; Alexey ; Myasoedova ; Nina ; Pozdnyakova ; Natalia ; Golovleva ; Ludmila
• 关键词：White rot fungus ; Laccase ; Redox-mediator ; Absorption spectra ; Lignin ; ABTS ; 2&prime ; -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) ; AAS ; atomic absorption spectroscopy ; EPR ; electron paramagnetic resonance ; MS ; mass spectrometry ; PAGE ; polyacrylamid
• 刊名：FEBS Letters
• 出版年：1997
• 出版时间：August 25, 1997
• 年：1997
• 卷：413
• 期：3
• 页码：446-448
• 全文大小：301 K

文摘

Yellow and blue forms of laccase from solid-state and submerged cultures of Panus tigrinus were isolated. Both laccases had similar molecular masses and specific activity, but yellow laccase had no `blue' maximum in the absorption spectrum. Blue laccase oxidized veratryl alcohol and a non-phenolic dimeric lignin model compound only in the presence of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) as electron-transfer mediator. Yellow laccase catalyzed these reactions without any additional compounds. It is supposed that yellow laccase is formed as a result of blue laccase modification by products of lignin degradation. These compounds might play a role of natural electron-transfer mediators for the oxidation of non-phenolic substances, catalyzed by yellow laccase.