The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C-terminal domains localize it to the cell wall

详细信息    查看全文

• 作者：Shweta Chatrath ; Vineet Kumar Gupta ; Lalit C. Garg ; ^{lalit@nii.ac.in" class="auth_mail}
• 关键词：PE_PGRS30 ; Localization ; PE domain ; PGRS domain ; C-terminal domain ; Mycobacterium tuberculosis
• 刊名：FEBS Letters
• 出版年：18 March, 2014
• 年：2014
• 卷：588
• 期：6
• 页码：990-994
• 全文大小：661 K

文摘

PE_PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE_PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE_PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C-terminal domain present in few PE_PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE_PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE_PGRS30.