Variables influencing the efficiency and interpretation of reverse transcription quantitative PCR (RT-qPCR): An empirical study using Bacteriophage MS2

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• 作者：Jaclyn A. Miranda ; Grieg F. Steward ; ^{grieg@hawaii.edu}
• 关键词：RT-qPCR ; reverse transcription real-time quantitative PCR ; RT ; reverse transcription ; rRNA ; ribosomal RNA ; mRNA ; messenger RNA ; cDNA ; complementary DNA ; s.d. ; standard deviation ; Cq ; quantitation cycle ; MMLV ; Moloney murine leukemia virus ; AMV ; avian myeloblastosis virus
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：1-10
• 全文大小：996 K
• 卷排序：241

文摘

Increasing total RNA and primer concentrations improved RT efficiency. At high concentrations, random hexamers performed as well as gene-specific primers. RNA standards corrected for residual systematic variability in RT efficiency. Length of the RNA standard dramatically influenced quantification accuracy.