Expression of heat shock proteins (HSPs) in Aedes aegypti (L) and Aedes albopictus (Skuse) (Diptera: Culicidae) larvae in response to thermal stress

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• 作者：Arun Sivan ; Ananganallur Nagarajan Shriram ; ^{shriraman@icmr.org.in} ; ^{anshriram@gmail.com} ; ^{anshriram@gmail.com} ; Nagarajan Muruganandam ; Ramanathan Thamizhmani
• 关键词：Heat shock proteins ; Aedes aegypti ; Aedes albopictus ; Relative quantification ; Larvae
• 刊名：Acta Tropica
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：167
• 期：Complete
• 页码：121-127
• 全文大小：908 K
• 卷排序：167

文摘

Quantitative real-time polymerase chain reaction was performed to assess expression levels of HSPs in response to thermal stress in the Andaman population of Ae. aegypti and Ae. albopictus under laboratory and in actual field conditions. Enhanced up-regulation of HSPs was evident in third instar larvae of Ae. aegypti with rise in water temperatures (31 °C, 33 °C, 34 °C) in the containers in the nature and thermally stressed (37 °C and 39 °C) in laboratory conditions. In Ae. albopictus up-regulation of HSPs was observed in field conditions at 34 °C only and when thermally treated at 37 °C, while down regulation was evident in larvae subjected to thermal stress in laboratory at 39 °C. The difference in expression levels of AeaHsp26, AeaHsc70 and AeaHsp83 genes in Ae. albopictus larvae was statistically significant between different exposure temperatures. All of these genes were significantly up-regulated at 37 °C. AeaHsp26, AeaHsc70 and AeaHsp83 are important markers of stress and perhaps function as proteins conferring protection and enhance survival in both the Aedes species.