Surface gene mutations of hepatitis B virus among high-risk patients with occult hepatitis B virus infection

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• 作者：Rasool Hamkar ; Arezoo Aghakhani ; Safyeh Soufian ; Mohammad Banifazl ; Nastaran Ghavami ; Mahsa Nadri ; Masoomeh Sofian ; Farrokhlagha Ahmadi ; Effat Razeghi ; Ali Eslamifar ; Amitis Ramezani
• 关键词：Occult hepatitis B virus (HBV) infection ; S mutation ; Sequencing
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：66
• 期：3
• 页码：285-291
• 全文大小：756 K

文摘

Surface gene mutants of hepatitis B virus (HBV) have been reported in a variety of patient groups. Because of limited data regarding these mutations in patients with occult HBV infections; we aimed to determine these mutations among high-risk patients with occult HBV infection. The presence of HBV-DNA was determined in patients with isolated anti-HBc by real-time polymerase chain reaction (PCR). Then, surface gene region was amplified by nested PCR and mutations were analyzed after sequencing. The mutations that resulted in nonfunctional hepatitis B surface antigen (HBsAg) were insertion of single nucleotide in 2 cases, which causes frameshift and single-nucleotide replacement, and premature stop codons at Leu15 and Gly10 in the other 2 cases. Amino acid substitution at amino acid position 207(S207N) was found in the other isolates. Our study suggested that “a” region mutations did not play a major role in HBsAg detection, and other genetic and nongenetic factors may be responsible for failure to detect HBsAg by routine laboratory tests.