Injectable adipose tissue combined with stem cells for soft-tissue augmentation: A pilot study for dental applications

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• 作者：Li-Ting Li^a ; Kuang-Ta Yao^b ; Shou-Cheng Teng^c ; Tiffany P. Sun^d ; Ching-Kuo Chen^a ; Chia-Chun Chen^a ; Ming-Lun Hsu^e ; ^{mlhsu@ym.edu.tw} ; Hsu-Wei Fang^a ; ^f ; ^{hwfang@ntut.edu.tw}
• 关键词：adipose-derived stem cells ; alveolar bone resorption ; cell-assisted lipotransfer ; ridge defects ; stromal vascular fraction cells
• 刊名：Journal of Dental Sciences
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：11
• 期：4
• 页码：377-386
• 全文大小：3018 K
• 卷排序：11

文摘

Bone resorption and soft-tissue defects are the typical physiologic responses after tooth extraction. Various dental ridge augmentation techniques have been applied and lack of the soft tissue is the major factor causing the failure. We propose that the adipose-derived stem cell can be useful in soft-tissue augmentation in dental applications. The objective of this study was to optimize the operation procedures for the isolation of adipose stem cells and tissues. Accelerated clinical protocols for effective transplantation of adipose tissue with high amount of adipose stem cells shall be developed.Materials and methodsOperation parameters were designed and optimized for the extraction of adipose tissue-derived stromal vascular cells. The optimized accelerated procedure was washing the lipoaspirate samples one time. Collagenase was then added and samples were incubated in a water bath for 30 minutes at 37°C and centrifuged at 1200g for 3 minutes. A mouse animal model was applied to evaluate the soft-tissue-filling effects using the optimized procedure.ResultsThe animal model tests demonstrated the filling and regeneration of the soft tissues with significant angiogenesis.ConclusionThis pilot study demonstrated the feasibility of soft-tissue augmentation applications.