Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools

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Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools

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作者：Marco Schü ; tz¹ ; ⁴ ; Alexander Batyuk¹ ; Christoph Klenk¹ ; Lutz Kummer¹ ; ⁴ ; Seymour de Picciotto² ; Basri Gü ; lbakan³ ; ⁵ ; Yufan Wu¹ ; Gregory A. Newby¹ ; ⁶ ; Franziska Zosel¹ ; Jendrik Schö ; ppe¹ ; Erik Sedlá ; k¹ ; ⁷ ; Peer R.E. Mittl¹ ; Renato Zenobi³ ; K. Dane Wittrup² ; Andreas Plü ; ckthun¹ ; ^{plueckthun@bioc.uzh.ch" class="auth_mail" title="E-mail the corresponding author}
关键词：DARPin ; designed ankyrin repeat protein ; nanoESI-MS ; nanoelectrospray ionization mass spectrometry ; MS ; mass spectrometry ; FACS ; fluorescence-activated cell sorting ; FAP ; fluorogen-activating protein ; GFP ; green fluorescent protein ; MG ; malachite green ; scFv ; single-chain variable fragment ; ORF ; open reading frame
刊名：Journal of Molecular Biology
出版年：2016
出版时间：27 March 2016
年：2016
卷：428
期：6
页码：1272-1289
全文大小：1743 K

文摘

•: Fluorogen-activating proteins are a novel class of fluorescent probes.
•: We created a fluorogen-activating DARPin (FADA) for malachite green.
•: Structural investigations revealed that the fluorogen is activated by a FADA dimer.
•: The FADA can be used as biosensors in extracellular and intracellular applications.
•: A prototype sensor that detects a presence of another molecule has been built.

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