Carotenoid composition of berries and leaves from six Romanian sea buckthorn (Hippophae rhamnoides L.) varieties

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• 作者：Raluca Maria Pop ; Yannick Weesepoel ; Carmen Socaciu ; Adela Pintea ; Jean-Paul Vincken ; Harry Gruppen
• 关键词：Carotenoid esters ; Chlorophylls ; Principal component analysis ; Electrospray ionization mass spectrometry ; Chemotaxonomic classification
• 刊名：Food Chemistry
• 出版年：15 March, 2014
• 年：2014
• 卷：147
• 期：Complete
• 页码：1-9
• 全文大小：1157 K

文摘

Berries and leaves from six varieties of Carpathians鈥?sea buckthorn (Hippophae rhamnoides L., ssp. Carpatica) were analysed for their carotenoid composition (free and esterified) using a combination of HPLC-PAD, GC-MS and UHPLC-PAD-ESI-MS techniques. GC-MS techniques revealed the fatty acid profile specific for each berry variety, while targeted UHPLC-MS analysis identified the fatty acids involved in carotenoids esterification: palmitic (C_16:0), myristic (C_14:0) and stearic (C_18:0). Total carotenoid content varied between 53 and 97 mg/100 g dry weight in berries, and between 3.5 and 4.2 mg/100 g DW in leaves. The carotenoid di-esters represented the main fraction among berry varieties having zeaxanthin di-palmitate as major compound, while leaves contained only free carotenoids like lutein, 尾-carotene, violaxanthin and neoxanthin. Principal component analysis identified the suitable carotenoid biomarkers characteristic for the Carpathians鈥?sea buckthorn from Romania with contribution to their taxonomic classification and authenticity recognition.