Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance

详细信息    查看全文

• 作者：Fahsai Kantawong ; Richard Burchmore ; Chris D.W. Wilkinson ; Richard O.C. Oreffo ; Matthew J. Dalby
• 关键词：Tissue engineering ; Surface topography ; Contact guidance ; Osteoprogenitor cells ; Proteomics
• 刊名：Acta Biomaterialia
• 出版年：2009
• 出版时间：May 2009
• 年：2009
• 卷：5
• 期：4
• 页码：1137-1146
• 全文大小：1189 K

文摘

We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype.