In vivo imaging of DNA double-strand break induced telomere mobility during alternative lengthening of telomeres

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• 作者：Nam Woo Cho^a ; ^{namwcho@mail.med.upenn.edu} ; Michael A. Lampson^c ; Roger A. Greenberg^a ; ^b ; ^{rogergr@mail.med.upenn.edu}
• 关键词：ALT ; alternative lengthening of telomeres ; HR ; homologous recombination ; DSB ; double-strand break ; MSD ; mean square displacement ; ER ; modified estrogen receptor domain ; DD ; destabilization domain ; 4-OHT ; 4-hydroxytamoxifen
• 刊名：Methods
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：114
• 期：Complete
• 页码：54-59
• 全文大小：798 K
• 卷排序：114

文摘

mCherry-ER-DD-TRF1-FokI is used to visualize DSB-induced telomere mobility. DSB generation and tracking are performed using a single protein. Transfection and imaging can be accomplished in as little as 48 h. Telomere mobility can be analyzed quantitatively using a semi-automated workflow.