Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes - A new assay suitable for bench and bedside conditions

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Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes - A new assay suitable for bench and bedside conditions

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作者：Tiago Granja ; ^{tiago.granja@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Jessica Schad ^{jessica.schad@student.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Patricia Schü ; ssel ^{patricia.schuessel@student.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Claudius Fischer ^{Claudius.Fischer@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Helene Hä ; berle ^{Helene.Haeberle@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Peter Rosenberger ^{peter.rosenberger@med.uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author} ; Andreas Straub ^{andreas.straub@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author}
关键词：ab ; antibody ; ADP ; Adenosine diphosphate ; APC ; Allophycocyanin Conjugate ; BV421 ; Brilliant Violet 421 ; CD ; Cluster of Differentiation ; COSIMA ; Coagulation and Systemic Inflammation During Mechanical Circulatory Assist ; ECC ; Extracorporeal circulation ; ECLS ; Extracorporeal life support ; ECMO ; Extracorporeal membrane oxygenation ; FITC ; Fluorescein isothiocyanate ; FSC ; Forward scatter ; GP ; Glycoprotein ; HIT ; Heparin-induced thrombocytopenia ; LIBS ; Ligand induced binding site ; LPS ; Lipopolysacharide
刊名：Thrombosis Research
出版年：2015
出版时间：October 2015
年：2015
卷：136
期：4
页码：786-796
全文大小：1311 K

文摘

•: Platelet leukocyte complexes are key mediators in thromboinflammatory disorders.
•: We describe a new flow cytometry assay to analyze platelet leukocyte interaction.
•: Platelet and leukocyte activation is measured using a six-colour approach.
•: The sensitivity of the assay after stimulation with various agonists is shown.
•: The applicability of this assay for bench and bedside analyses is demonstrated.