Tuber borchii fruit body: 2-dimensional profile and protein identification

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• 作者：Pierleoni ; Raffaella ; Buffalini ; Michele ; Vallorani ; Luciana ; Guidi ; Chiara ; Zeppa ; Sabrina ; et. al.
• 关键词：Tuber borchii ; Ectomycorrhizal fungi ; Fruit body maturation ; Edman degradation ; Mass spectrometry
• 刊名：Phytochemistry
• 出版年：2004
• 出版时间：April, 2004
• 年：2004
• 卷：65
• 期：7
• 页码：813-820
• 全文大小：269 K

文摘

The formation of the fruit body represents the final phase of the ectomycorrhizal fungus T. borchii life cycle. Very little is known concerning the molecular and biochemical processes involved in the fructification phase. 2-DE maps of unripe and ripe ascocarps revealed different protein expression levels and the comparison of the electropherograms led to the identification of specific proteins for each developmental phase. Associating micropreparative 2-DE to microchemical approaches, such as N-terminal sequencing and 2-D gel-electrophoresis mass-spectrometry, proteins playing pivotal roles in truffle physiology were identified.